Documentation

CrisPRO.ai Documentation

Everything you need to transform therapeutic development with AI-powered intelligence

Browse APIs Try Variant Impact

API Reference

10 endpoints available

Oracle APIs (Discriminative AI)

5 endpoints

Predict Variant Impact

/predict_variant_impact

Predicts the functional and clinical impact of any genetic variant (SNVs, indels, structural variant...

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Context-Aware Gene Importance Prediction

/predict_gene_essentiality

Predicts whether a given gene is essential for cell survival or proliferation in a specific cellular...

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Direct Guide RNA Cutting Efficiency Prediction

/predict_crispr_spacer_efficacy

Predicts the on-target functional efficacy (likelihood of successful cutting/activity) of a CRISPR g...

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Epigenomic Context Prediction

/predict_chromatin_accessibility

Predicts the chromatin accessibility state (e.g., open, closed, accessible) of a given genomic regio...

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Protein-Level Functional Impact

/predict_protein_functionality_change

Predicts the change in a protein's overall function, stability, or binding affinity given its sequen...

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Forge APIs (Generative AI)

5 endpoints

AI-Powered Guide Design

/generate_optimized_guide_rna

Generates novel or optimized guide RNA sequences for a specific genomic target, tailored for a thera...

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HDR Template Optimization

/generate_repair_template

Generates optimized DNA repair template sequences for homology-directed repair (HDR) applications (e...

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Context-Aware Sequence Design

/generate_epigenome_optimized_sequence

Generates DNA sequences (e.g., regulatory elements, or even parts of guide RNAs/repair templates) th...

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Targeted Expression Control

/generate_optimized_regulatory_element

Generates novel or modified promoter, enhancer, or other regulatory sequences to achieve desired gen...

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Functional Protein Design

/generate_therapeutic_protein_coding_sequence

Generates DNA coding sequences for novel or modified therapeutic proteins with specific desired func...

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Products

Oracle

95.7% AUROC

Zero-shot variant impact prediction with biological reasoning

/predict_variant_impact/predict_gene_essentiality/predict_crispr_spacer_efficacy

Forge

70% Pfam-hit rate

Agentic therapeutic design for cancer immunotherapies

/generate_optimized_guide_rna/generate_repair_template/generate_epigenome_optimized_sequence

Boltz

83% confidence

3D structural validation and binding affinity prediction

Coming soon

Command Center

Full audit trail

Workflow orchestration with provenance tracking

Coming soon

Use Cases

Synthetic Lethality & Essentiality Agent

Clinical • Intermediate

Identify double-hit vulnerabilities and score gene essentiality for precision drug targeting. When cancer cells lose one pathway (e.g., HR), they become dependent on backup pathways (e.g., PARP) - we identify these dependencies and target them with precision drugs.

50% drug match accuracy100% Evo2 usage✅ Implementation Complete

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API Reference

Complete endpoint documentation

Synthetic Lethality

Use case: Identify double-hit vulnerabilities

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CrisPRO.ai Documentation

API Reference

Oracle APIs (Discriminative AI)

Predict Variant Impact

Context-Aware Gene Importance Prediction

Direct Guide RNA Cutting Efficiency Prediction

Epigenomic Context Prediction

Protein-Level Functional Impact

Forge APIs (Generative AI)

AI-Powered Guide Design

HDR Template Optimization

Context-Aware Sequence Design

Targeted Expression Control

Functional Protein Design

Products

Oracle

Forge

Boltz

Command Center

Use Cases

Synthetic Lethality & Essentiality Agent

Quick Links

API Reference

Synthetic Lethality

Popular Questions

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