Overview

Workspace

Metastatic Cascade Lock

Metastatic Cascade
Log
[0]L1_TARGET_STANDBY
[1]MAPPING_CASCADE_STEPS
[2]READY_FOR_INTERCEPT
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AF3 Guide

TWIST1_10

Target

TWIST1

pLDDT

67.9

iPTM

0.38

AF3 PASSTWIST1 · structure
Lock gene

TWIST1

AF3 guide

TWIST1_10

Target-Lock

Impact

High

Lock invasion drivers before basement membrane exit (MMP2/MMP9 axis).

Mechanism Detail

Local Invasion

EMT · ECM breach

Lock invasion drivers before basement membrane exit (MMP2/MMP9 axis).

AF3 RNA–DNA gate
pLDDT ≥5067.9
iPTM ≥0.30.38

Cohort 15/15 pass · 96nt gRNA + 60bp DNA

L1 Target-Lock ranks drivers on 38 genes × 8 steps (Evo2 + Enformer). Structural triage is pre-synthesis only — not cleavage validated.